研究内容
- 针对植物学领域中存在的外源分子递送及植物遗传转化两大瓶颈问题，开发了一系列基于纳米材料（碳纳米管、DNA纳米结构、金纳米颗粒）的载体，将质粒DNA、siRNA等成功递送至植物细胞中并发挥相应生物学功能，成功攻克了植物体系外源分子递送的瓶颈问题；在此基础上，深入系统的研究了纳米载体与植物组织、细胞的相互作用，发现了植物体系RNA递送的新机制，为植物体制中纳米载体的设计提供了技术支持。
- 在前期的研究基础上，本课题组将以DNA折纸术等生物可编程技术为核心，实现对植物体生命活动的调控及监测，助力精准生物育种。 研究包括但不限于以下方向：
- 框架核酸的精密设计和可控自组装；
- 纳米探针开发及植物体系传感和成像应用；
- 纳米技术介导的植物体系基因编辑、功能分子递送和疾病治疗；
承担项目
- 国家重点研发计划课题
- 国家自然科学基金面上项目
- 上海市浦江人才
- 上海市科委合成生物中心概念验证项目
论文
- Huan Zhang#, Natalie S Goh#, Jeffrey Wang, Gozde S Demirer, Salwan Butrus, So-Jung Park, Markita Landry*. Nanoparticle Cellular Internalization is Not Required for RNA Delivery to Mature Plant Leaves. Nature Nanotechnology, 2022, 17, 197-205.
- Yi Xu#, Yao Luo#, Xiaoyun Lu#, Jingyi Ye, Zeyu Chen, Yao Hu, Chen Shen, Bin Zhao, Erfeng Kou, Jie Deng, Chunhai Fan, Huan Zhang*, Honglu Zhang*. Dynamic modulation of multicellular interactions via ATP-dissipative DNA assembly. JACS, 2025, 147,28277−28288.
- Huan Zhang#, Honglu Zhang#, Gozde S. Demirer, Eduardo González-Grandío, Chunhai Fan and Markita P. Landry*. Engineering DNA nanostructures for siRNA delivery in plants. Nature Protocols, 2020, 15, 3064-3087.
- Zeyu Chen#, Xu Chen#, Dan Lu, Huating Kong, Jingyi Ye, Chunhai Fan, Honglu Zhang,*and Huan Zhang*.Engineering Liquid Hierarchical Materials with DNA-Programmed Spherical Nucleic Acids. Advanced Science, 2025, 12,e04471 (1-12).
- Huan Zhang#, Gozde S. Demirer#, Honglu Zhang#, Tianzheng Ye, Natalie S. Goh, Abhishek J. Aditham, Francis J. Cunningham, Chunhai Fan and Markita P. Landry*. DNA Nanostructures Coordinate Gene Silencing in Mature Plants. PNAS, 2019,116, 15:7543-7548.
- Huan Zhang#, Yuhong Cao#, Dawei Xu, Natalie S Goh, Gozde S Demirer, Stefano Cestellos-Blanco, Yuan Chen, Markita P. Landry*, Peidong Yang*. Gold Nanocluster Mediated Delivery of siRNA to Intact Plant Cells for Efficient Gene Knockdown. Nano Letters, 2021, 21, 5859–5866.
- Erfeng Kou; Zhongxu Luo; Jingyi Ye; Xu Chen; Dan Lu; Markita P. Landry; Honglu Zhang; Huan Zhang*. Sunlight-Sensitive Carbon Dots for Plant Immunity Priming and Pathogen Defense. Plant Biotechnology Journal, 2025, 1-12.
- Gozde S. Demirer#, Huan Zhang#, Natalie S. Goh, Markita P. Landry*. Carbon nanotube-mediated DNA delivery without transgene integration in intact plants. Nature Protocols, 2019, 14, 2954-2971.
- Honglu Zhang#, Yu Wang#, Huan Zhang#, Xiaoguo Liu#, Antony Lee, Jie Chao, Huajie Liu, Jiang li, Jiye Shi, Lihua Wang, Lianhui Wang, Xiaoyu Cao, Carlos Bustamante, Zhongqun Tian*, Chunhai Fan*. Programming chain-growth co-polymerization of DNA hairpin tiles for in-vitro hierarchical supramolecular organization. Nature Communications, 2019, 10, 1006 (1-11).
- Gozde S. Demirer, Huan Zhang, Juliana L. Matos, Natalie Goh, Francis Cunningham, Younghun Sung, Roger Chang, Abhishek J. Aditham, Linda Chio, Myeong-Je Cho, Brian Staskawicz and Markita P. Landry*. High Aspect Ratio Nanomaterials Enable Delivery of Functional Genetic Material Without Transgenic DNA Integration in Mature Plants. Nature Nanotechnology, 2019, 14, 456-464.
- Gozde S. Demirer, Huan Zhang, Natalie S. Goh and Markita P. Landry. Nanotubes effectively deliver siRNA to intact plant cells and protect siRNA against nuclease degradation. Science Advances, 2020, 6, eaaz0495.
- Qianqian Hu#, Erfeng Kou#, Xiuzhen Liao, Ruiyi Qiu, Qi Tang, Huan Zhang,* Yun Zheng,* Ji Yang,* and Binglian Zheng*. Nanoparticle Delivery of Antisense miR162 Inhibits Invasive Habitat Adaption of Alternanthera Philoxeroides. Advanced Science, 2025, 2416747.
- Xiaoyue Xu, Erfeng Kou, Kunyu Zhang*, Honglu Zhang*, Huan Zhang*; Programmable DNA-based biomaterials for bone tissue engineering, Fundamental Research, 2025, 5, 1384–1400.
- Bin Zhao, Jingyi Ye, Zeyu Chen, Danyang Chen, Jiahui Chai, Zhongxu Luo, Xu Chen, Dan Shao, Qianjun He*, Chunhai Fan, Huan Zhang*, Honglu Zhang*. Mitochondria-Targeted Photoredox Catalysis Activates Pyroptosis for Effective Tumor Therapy. Advanced Functional Materials, 2024, 2417681.
- Zeyu Chen#, Xu Chen#, Bin Zhao, Honglu Zhang*, Huan Zhang*. Efficient Poly-Adenine-Tailed DNA Functionalization of Gold Nanorods for Tailored Nanostructure Assembly. The Journal of Physical Chemistry Letters, 2024, 15, 4400-4407.
- Yao Hu, Zhongxu Luo, Zhilei Ge, Qian Li, Peihui Yang, Honglu Zhang*and Huan Zhang*. Morphology Dictated Immune Activation with Framework Nucleic Acids. Small, 2023, 23034: 1-10.
- Jingyi Ye, Huan Zhang* and Honglu Zhang*. In situ DNA storage with whole blood cryogenic silicification. Matter, 2023, 6, 26-29.
- Bin Zhao; Zhongxu Luo; Honglu Zhang*; Huan Zhang*; Imaging tools for plant nanobiotechnology, Frontiers in Genome Editing, 2022, 4: 1029944.
专利著作
- 一种具有光催化杀菌和免疫诱导功能的碳点及其制备方法和应用
- 一种制备金属液滴的方法
- 一种具有近红外光催化性能的富含空穴的氧化钼纳米片及其制备方法和应用
- 一种基于 CRISPR 技术的 eccDNA 快速检测方法
学术活动
- 1) (Invited talk) Huan Zhang*, Chunhai Fan; Programmable DNA Nanomaterial Enabled Delivery in Mature Plants; International Congress on Nanomedicine and Nanobiotechnology, 河南开封，2024-12-6至2024-12-8。
- 2) （邀请报告）张欢; 樊春海; 核酸纳米材料的RNA递送研究，纳米生物学与纳米生物材料2024年崖州会议，海南三亚，2024-11-22至2024-11-27。
- 3) （邀请报告）张欢; 樊春海; 可编程核酸纳米材料的RNA递送研究，2024广州分子工程+国际峰会，广东广州，2024 -11-8至2024 -11-11。
- 4) (Invited Talk) Huan Zhang; Chunhai Fan; Markita Landry; Nanotechnology Enabled Genetic Engineering in Plants: NA Delivery in Mature Plants, 15th International Association for Plant Biotechnology
- Congress (IAPB 2023), 韩国大田, 2023-8-6 to 2023-8-11。
- 5) (Talk) Huan Zhang; Markita Landry; Chunhai Fan; DNA Nanomaterial Enabled RNA Delivery for Plant
- Genetic Engineering, The 10th International Conference on DNA Nanotechnology, 浙江杭州, 2023-9-
- 8至2023-9-10。
- 6) （口头报告）张欢; 樊春海; Markita Landry; 植物体系纳米载体的开发及RNA分子递送, 2023中国生物材料大会，重庆，2023-10-12至2023-10-15。
- 7) (Talk) DNA Nanostructures coordinate gene silencing in mature plants. The 25th International Conference on DNA Computing and Molecular Programming, Seattle, WA, August 2019.
- 8) (Talk) DNA nanostructures coordinate gene silencing in mature plants. ACS National Meeting, San Diego, CA, August 2019.
社会兼职
- Exploration 青年编委；
- 中国分析测试协会核酸分析分会 委员
荣誉
- 上海市浦江人才
- 2022杰出青年女性科技工作者