发布时间:2021-10-21  阅读次数:3961

报 告 人:潘 健   植物科学系 博士后

   间:2021年10月27日 (星期三) 12:40-14:00

   点:农生学院创新楼(B楼)104会议室

主办单位:农生学院学科与科技办、农生学院青年教师联谊会

 

 

报告题目:黄瓜性别决定的分子基础

报告摘要:

黄瓜是重要的园艺作物,同时也是研究高等植物花性别决定的模式植物。长期以来,乙烯被认为是促进雌花分化的关键因素。在乙烯的合成与调控网络中,“CsERF31-CsACS2”反馈调控的激活决定雌花而非两性花的决定;CsACS11CsACO2隐性突变决定植株为全雄,CsACS1的显性突变和CsWIP1的隐性突变决定植株为全雌。然而,现有的分子模型只能用于解释单性花植株的性别决定,为探究雌雄同株中的雌花与雄花如何自发地决定性别,我们通过QTL分析与近等基因系图位克隆,发现CsTCP18是控制强雄表型的候选基因。利用基因编辑证实CsTCP18的功能缺失造成植株全雄表型。生化分析显示CsTCP18通过调控CsPIN3,介导了叶腋处花芽向雌花的转化。同时,通过生物信息学,遗传群体分析,以及古籍查阅,发现CsTCP18位点在古代中国受到人工选择,即华北类型早雌花性状是中国农民长期驯化的结果。上述基因及其遗传关系,构成了目前黄瓜性别决定的分子调控网络。

报告人简介:

报告人先后于2011和2014年本科、硕士毕业于沈阳农业大学生物学专业,2018年获上海交通大学进行园艺学博士学位,2019年至今在上海交通大学园艺系进行博士后研究。

【课题组研究方向】:

黄瓜重要遗传资源的挖掘与应用。主要研究方向如下:

(1)黄瓜重要性状的基因挖掘; 

(2)黄瓜种质资源的创新与应用;

(3)水果与华南型黄瓜的分子育种。

 

ACADEMIC SALON (XXVIII)

SPEAKER: Jian Pan

Post Doctor,  Dept. of Plant Science

TIME :12:40-14:00  Oct. 27, 2021

VENUE:Room 104, Building B, SAB

ORGANIZER:Office of Discipline and Science & Technology, SAB;

Young Teachers Association, SAB

TITLE: The molecular basement of Cucumber Sex determination

ABSTRACT:

Cucumber (Cucumis sativus L.) is an important horticultural crop, but also a model plant to study the sex determination of embryophyte. Ethylene has long been considered a key factor in promoting female flower differentiation. In the synthesis and regulation network of ethylene, the activation of "CsERF31-CsACS2" feedback regulation determines the decision of female flowers rather than female flowers; CsACS11 and CsACO2 recessive mutations determine the plant to be androecy, the dominant mutation of CsACS1 and the recessive mutation of CsWIP1 to determine the plant to be gynoecy. However, the existing molecular model can only be used to explain the sex decision of monosexual flower plants, to explore how female and male flowers in the same plant spontaneously determine gender, we found that CsTCP18 is a candidate gene to control strong male phenotypes through QTL analysis and proximal gene line mapping cloning. Gene editing was used to confirm that the functional deficiency of CsTCP18 resulted in the plant's full male phenotype. Biochemical analysis showed that CsTCP18 mediated the transformation of buds from leaf buds to female flowers by regulating CsPIN3. At the same time, through bioinformatics, genetic population analysis, and ancient books, it is found that CsTCP18 sites were artificially selected in ancient China, that is, the early female flower traits of the North China type are the result of long-term domestication of Chinese farmers. The above-mentioned genes and their genetic relationship constitute the molecular regulatory network of cucumber sex determination.

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