Department of Animal Science
Research Interests:(1)Antibody engineering (2)Applying ecology technique for preventing animal disease (3)Prevention of Zoonosis
Address:Room 3-219, Agriculture and Biology Building




ResumeZhu Jianguo, male, born in 1962, PH.D, Visiting Scholar studied in University of Michigan, Professor of Shanghai Jiaotong University, mentor of Ph.D student , research interests is focused on Application of gene engineering and antibody engineering for prevention and control of animal disease and zoonosis.
From 1984 to 1992, worked as a veterinary medicine technician for eight years, taking charge of prevention and control of Bovis Tuberculosis and Brucella infection ;
From 1992 to 1995, studied as a graduated student in Veterinary college, Yangzhou University, China. The Master thesis titled "Research in Monoclonal Antibody of IBV";
From 1995 to 1998, was assigned as an associated professor in Zhengzhou College of Animal Husbandry Engineering(ZZCAH), rewarded as excellent young teacher in 1997;
From 1998 to 2001, studied as a Ph.D candidate in graduate school of Chinese Academy of Agricultural Science(CAAS) , majored in application of molecular biology for preventing and controlling animal disease , the thesis titled " Research of sexual specific expressive gene and its function of Schistosoma japonicum Chinese strain" From 2001 to 2008, was assigned as associated professor and mentor of master student in the Department of Veterinary, Shanghai Jiaotong University ;
From 2007 to 2008, studied as a visiting scholar in Department of immunology and Microbiology, University of Michigan;
Since 2008, have been assigned as professor and mentor of Ph.D student in Shanghai Jiaotong University .
I took part in the works to compile some teaching books for bachelor students major in prevention and control of animal disease, such as "Quarantine of Food Microbiology" "Quarantine of food sanitation" " Veterinary Pathology" and other programming books of high agricultural college. I am now teaching some courses for different students , such as "Veterinary Pathology" and "Preventive Veterinary" for undergraduates; "Modern Immunity" and "Zoonosis & Public Health" for graduated students; "Advance of Research in Zoonosis" and "Molecule Immunity" for Ph.D students. Since being assigned as mentor of graduated student in 2002, I have being directed 15 graduated students, eleven of whom have gotten Master degree, one of whom obtained the honor of Chinese national excellent dissertation of Master degree, and the other one gotten a honor of Shanghai excellent dissertation of Master degree. I also gotten 1 third class honor of reforming project in teaching.



Researching project

Our primary research interest is to understand pathogenesis and immunology of infectious diseases and develop vaccines and antibody against pathogenesis. Much of my academic achievements can be credited to my solid command of a wide variety of basic principle and skills in immunology, microbiology and molecular biology such as Recombinant Antibody, Monoclonal Antibody (McAb) , McAb-based ELISA, McAb-based Immunofluoresence, PCR , gene knockout and other molecular techniques. After finished the research projects " Research of standard examination method of AIV from animal product" and "study on the standard way to detect campylobacter jejuni in animal products ", we have established two standard methods such as RT-PCR way to detect avian influence virus in animal products , and Real time–PCR way to detect Campylobacter jejuni in animal products , both of which have been certificated by the authorities ; When the projects "Research of fast detection and cure of Kidney type of IBV" and" Development of novel monoclonal diagnosis kit for IBV" were finished, I developed the RT-Nest-PCR method for detection of IBV in infected chicken tissues, which have been used in the animal model research program for study the infection mechanism of coronavirus. I also took part in and finished the two National High Technology Research and Development Program("863"Program) of China, including "Study on the key measure to prevent and control Eperythrozoon" , and "Study on the DNA vaccine of Shistosoma japonicum"; I also directed and finished two projects "Study on the mainly immunogenicity proteins of Mycobacterium bovis " and "Application of synthesized measure to prevent and control Mycobacterium bovis in Ningxia area", six secreted proteins in Mycobacterium tuberculosis have been identified and proved to have significant antigenicity, they have been further used to develop the indirect ELISA to detect serum antibody of infected animals; I am now directing the projects "Study on the biological pharmaceutical to prevent and treat mastitis of milk cow " and "Raybiotech-SJTU antibody engineering research", 6 of scFv antibodies against IBV have been identified and further function study of them are being ongoing.



Achievement and award:

I undertake researching one National High Technology Research and Development Program("863"Program) of China,"Study on the key measure to prevent and control Eperythrozoon" , obtaining the second award of scientific and technological progress of Chinese Ministry of Education ; I also undertake researching other  National High Technology Research and Development Program("863"Program) of China "Study on the DNA vaccine of Shistosoma japonicum"; obtaining the first prize of scientific and technological progress of Chinese Academy of Agricultural Sciences; I have had more than 100 papers published , including16 SCI cited and 4 EI cited papers . I also gotten 8 patents authorized ny china .



Main publish paper:

1 Man Wang, Yan Zhang, Jianguo Zhu *,Anti-Staphylococcus aureus single-chain variable region fragments provide protection against mastitis in mice, Applied Microbiology and Biotechnology,2016, 100(5) 2153-2162 

2 Zhang Y, Wang M,  Yu XQ, Ye CR, Zhu JG, Analysis of polymorphisms in the FUT1 and TAP1 genes and their influence on immune performance in Pudong White pigs. Genet Mol Res. 2015 , 14(4):17193-203

3 Lin Yuan, Li Benqiang, Ye Jiaxin, Wang Man, Wang Jianhua, Zhang Ying, and Zhu Jianguo * ,Neutralization analyzing of chicken single-chain variable fragment derived from immune antibody library against infectious bronchitis viru. Viral Immunology. 2015, 28(7): 397-404

4  M. Wang ,Yan Zhang, B.Q. Li, J.G. Zhu *,Construction of scFv that bind both fibronectin- binding protein A and clumping factor A of Stapylococcus aureus ,Research in Veterinary Science  2015, 100:109–114.

5  Benqiang Li , Jiaxin Ye , Yuan Lin , Man Wang , Jianguo Zhu * , Preparation and identification of a single-chain variable fragment antibody against Newcastle diseases virus F48E9, Veterinary Immunology and Immunopathology 161 (2014) 258–264

6 Benqiang Li , Jiaxin Ye , Yuan Lin , Man Wang , Rui Jia , Jianguo Zhu *, Selection and characterization of single-chain recombinant antibodies against phosphoprotein of newcastle disease virus , Biologicals 42 (2014) 285-289

Jianguo Zhu ,et al ,Intrabody-based gene therapeutic strategy, Reviews in Medical Microbiology.2012,23(4):76-81

8 Jianguo Zhu et al,Production and properties of single domain antibody fragments ,Reviews in Medical Microbiology.2012,23(2):9-13

9 Fang Liu,Yuan  Lin, Jianguo Zhu*,The primarily use in indirect ELISA of secreted proteins Mb1761c and Mb2277 of  M.bovis,Journal of Immunoassay and Immunochemistry ,2012 Jul;33(3):269-74

10  Jianguo Zhu, Charles Bradford Larson, Megan Ann Ramaker, Kimberly Quandt, Jered Wendte, Kimberly Ku, Fang Chen, George Jourdian, Ramesh Vemulapalli, Gerhardt G. Schurig,,Yongqun “Oliver”He, Characterization of recombinant B. abortus strain RB51SOD towards  understanding the uncorrelated innate and adaptive immune responses induced by RB51SOD compared to its parent vaccine strain RB51,CELLULAR AND INFECTION MICROBIOLOGY.2011(11):1-10

11 Yuan  Lin, Jianguo Zhu*,Improved Affinity of a Chicken Single-chain Antibody to Avian Infectious Bronchitis Virus by Site-directed Mutagenesis of Complementarity-determining Region H3, African Journal of Biotechnology,2011, 10(79), 18294-18302

12 Jianguo Zhu,Yuanlin,Surveillance of infection of Mycobacterium bovis,Reviews in Medical  Microbiology. 2011, 22(2):17-21

13 Jianguo Zhu, et al, Potential use of single-chain Fv proteins for human and animal health, Reviews in Medical Microbiology,2010,21(4) 103-108

14 Jian-guo Zhu,et al, Epidemiology of hepatitis E. Reviews in Medical Microbiology,2009 ,19(4) 98-103(SCI).

15 Jian-Guo Zhu, et al , The virulence determinants of Campylobacter jejuni and its ability to colonize hosts. Reviews in Medical Microbiology,2008 ,19(1)13-18 (SCI).

16 Jian-Guo Zhu ,et al ,Analysis of similarity of S1 gene of  Infectious bronchitis virus isolates in Shanghai China ,Archivos de medicina veterinaria. 2007,39(3)223 -228 (SCI);

17 ZHU Jian-Guo et al, Studies on DNA vaccine of the conservative region of gene encoding the male-specific  Gynecophoral canal protein of Schistosoma japonicum (Chinese strain) [J].Journal of Shanghai Jiaotong university,2006(EI)  

18 Zhu Jian-Guo  et al,Antibiotic resistance in Campylobacter,  Reviews in Medical Microbiology,2006, 17(4):107-112(SCI)

19 Zhu Jian-Guo, et al,Molecular mechanisms of pathogenesis of Campylobacter jejuni , Reviews in Medical Microbiology,2006;17(2):39-43, (SCI);

20 Qian DH, Zhu GJ, ,(2006)Isolation and characterization of a coronavirus from pigeons with pancreatitis  Am J Vet Res.2006; 67(9):1575- (SCI);

21 朱建国等,日本血吸虫中国大陆株肌动蛋白编码基因的克隆及其表达,生物化学与生物物理学报(SCI),2000,32(5):545-549

22. 林源,王建华,朱建国*,鸡传染性支气管炎病毒单链抗体的筛选及其互补决定区序列分析,西北农林科技大学学报,2010,39(2)47-53

23. 赵蕾,林源,李本强,朱建国*,基因工程小分子抗体及其在动物疾病研究中的应用,中国兽药杂志,2011,45(2)41-46

24. 赵蕾,林源,李本强,朱建国*,抗鸡传染性支气管炎病毒单链抗体间接ELISA筛选方法的建立,上海交通大学学报(农业科学版),2011,(2)64-68

*Corresponding author

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